Collect a fresh urine sample in a clean, dry container preferably glass. First morning samples contain the highest concentration of target markers. Remove one reagent strip from the bottle and immediately replace the container cap, minimizing the exposure of the remaining test strips to light and air. Completely immerse the reagent pads of the strip in the urine sample and then remove immediately to avoid dissolving out the reagent pads. A red line will appear next to the Control (C) region when the wick has absorbed sufficient urine While removing the reagent strip, run the edge of the strip against the rim of the specimen container to remove excess urine. Hold the strip in a horizontal position to prevent possible cross contamination of chemicals located in adjacent reagent pads. Place the cap back on and set the device on a flat level surface Compare the color change of reagent pads to the corresponding color chart on the bottle label. Read results according to the chart's time frame for each panel tested. Read results in minutes. Discuss with your physician or healthcare provider and then discard the used reagent strip.